Ipsita Datta, Ye Ma, and Ehsan Baharlou presented their research titled “Toolpath Informed Biofabrication” on November 6 at ACADIA 2025 in Miami, Florida. The project examines how robotic additive manufacturing can spatially guide mycelial growth within oyster shell biocomposites by treating toolpaths as biological interfaces. Through small and large scale experiments, the team evaluated several geometric filling strategies and demonstrated that Hilbert printing patterns encourage consistent hyphal colonization due to controlled porosity and continuous movement. Architectural scale panels were produced using updated paste extruders and an indirect inoculation system. The research advances living material fabrication by linking digital toolpath logic to metabolic behavior, proposing scalable methods for biointegrated construction.